5WLS

Crystal Structure of a Pollen Receptor Kinase 3

5WLS の概要

• エントリーDOI: 10.2210/pdb5wls/pdb
• 分子名称: Pollen receptor-like kinase 3, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: pollen receptor kinase, leucine rich repeat, extracellular domain, receptor like kinase, plant protein
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47537.93

構造登録者

Xu, G.,Chakraborty, S.,Pan, H. (登録日: 2017-07-27, 公開日: 2018-06-06, 最終更新日: 2024-10-30)

主引用文献

Chakraborty, S.,Pan, H.,Tang, Q.,Woolard, C.,Xu, G.
The Extracellular Domain of Pollen Receptor Kinase 3 is structurally similar to the SERK family of co-receptors.
Sci Rep, 8:2796-2796, 2018

PubMed Abstract: During reproduction in flowering plants, the male gametophyte delivers an immotile male gamete to the female gametophyte in the pistil by formation of pollen tubes. In Arabidopsis thaliana, two synergid cells situated on either side of the egg cell produce cysteine-rich chemoattractant peptide LURE that guides the pollen tube to the female gametophyte for sexual reproduction. Recently, in Arabidopsis thaliana, Pollen Receptor Kinase 3 (PRK3), along with PRK1, PRK6, and PRK8, have been predicted to be the receptors responsible for sensing LURE. These receptors belong to the Leucine Rich Repeat Receptor Like Kinases (LRR-RLKs), the largest family of receptor kinases found in Arabidopsis thaliana. How PRKs regulate the growth and development of the pollen tube remains elusive. In order to better understand the PRK-mediated signaling mechanism in pollen tube growth and guidance, we have determined the crystal structure of the extracellular domain (ecd) of PRK3 at 2.5 Å, which resembles the SERK family of plant co-receptors. The structure of ecdPRK3 is composed of a conserved surface that coincides with the conserved receptor-binding surface of the SERK family of co-receptors. Our structural analyses of PRK3 have provided a template for future functional studies of the PRK family of LRR-RLK receptors in the regulation of pollen tube development.

PubMed: 29434276
DOI: 10.1038/s41598-018-21218-y

実験手法

X-RAY DIFFRACTION (2.496 Å)