5WIP

TraE protein in complex with 2-(2-furyl)isonicotinic acid

5WIP の概要

• エントリーDOI: 10.2210/pdb5wip/pdb
• 分子名称: Conjugal transfer protein, 2-(furan-2-yl)pyridine-4-carboxylic acid (3 entities in total)
• 機能のキーワード: type iv secretion system, fragment-based drug design, complex, inhibitor, antimicrobial resistance, bacterial secretion, virb, protein transport
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 75461.58

構造登録者

Casu, B.,Arya, T.,Bessette, B.,Baron, C. (登録日: 2017-07-19, 公開日: 2017-11-15, 最終更新日: 2023-10-04)

主引用文献

Casu, B.,Arya, T.,Bessette, B.,Baron, C.
Fragment-based screening identifies novel targets for inhibitors of conjugative transfer of antimicrobial resistance by plasmid pKM101.
Sci Rep, 7:14907-14907, 2017

PubMed Abstract: The increasing frequency of antimicrobial resistance is a problem of global importance. Novel strategies are urgently needed to understand and inhibit antimicrobial resistance gene transmission that is mechanistically related to bacterial virulence functions. The conjugative transfer of plasmids by type IV secretion systems is a major contributor to antimicrobial resistance gene transfer. Here, we present a structure-based strategy to identify inhibitors of type IV secretion system-mediated bacterial conjugation. Using differential scanning fluorimetry we screened a fragment library and identified molecules that bind the essential TraE protein of the plasmid pKM101 conjugation machinery. Co-crystallization revealed that fragments bind two alternative sites of the protein and one of them is a novel inhibitor binding site. Based on the structural information on fragment binding we designed novel small molecules that have improved binding affinity. These molecules inhibit the dimerization of TraE, bind to both inhibitor binding sites on TraE and inhibit the conjugative transfer of plasmid pKM101. The strategy presented here is generally applicable for the structure-based design of inhibitors of antimicrobial resistance gene transfer and of bacterial virulence.

PubMed: 29097752
DOI: 10.1038/s41598-017-14953-1

実験手法

X-RAY DIFFRACTION (2.62 Å)