5WHW

Using sound pulses to solve the crystal harvesting bottleneck

Summary for 5WHW

• Entry DOI: 10.2210/pdb5whw/pdb
• Descriptor: Proteinase K, CALCIUM ION, NITRATE ION, ... (6 entities in total)
• Functional Keywords: crystal harvesting, drug discovery, acoustic droplet ejection, hydrolase
• Biological source: Parengyodontium album
• Total number of polymer chains: 1
• Total formula weight: 29450.33

Authors

Soares, A.S.,Brennan, H.M.,Natarajan, R.,McCarthy, L.,Leroy, L. (deposition date: 2017-07-18, release date: 2017-08-02, Last modification date: 2024-11-20)

Primary citation

Samara, Y.N.,Brennan, H.M.,McCarthy, L.,Bollard, M.T.,Laspina, D.,Wlodek, J.M.,Campos, S.L.,Natarajan, R.,Gofron, K.,McSweeney, S.,Soares, A.S.,Leroy, L.
Using sound pulses to solve the crystal-harvesting bottleneck.
Acta Crystallogr D Struct Biol, 74:986-999, 2018

PubMed Abstract: Crystal harvesting has proven to be difficult to automate and remains the rate-limiting step for many structure-determination and high-throughput screening projects. This has resulted in crystals being prepared more rapidly than they can be harvested for X-ray data collection. Fourth-generation synchrotrons will support extraordinarily rapid rates of data acquisition, putting further pressure on the crystal-harvesting bottleneck. Here, a simple solution is reported in which crystals can be acoustically harvested from slightly modified MiTeGen In Situ-1 crystallization plates. This technique uses an acoustic pulse to eject each crystal out of its crystallization well, through a short air column and onto a micro-mesh (improving on previous work, which required separately grown crystals to be transferred before harvesting). Crystals can be individually harvested or can be serially combined with a chemical library such as a fragment library.

PubMed: 30289409
DOI: 10.1107/S2059798318011506

Experimental method

X-RAY DIFFRACTION (1.71 Å)