5WHW

Using sound pulses to solve the crystal harvesting bottleneck

5WHW の概要

• エントリーDOI: 10.2210/pdb5whw/pdb
• 分子名称: Proteinase K, CALCIUM ION, NITRATE ION, ... (6 entities in total)
• 機能のキーワード: crystal harvesting, drug discovery, acoustic droplet ejection, hydrolase
• 由来する生物種: Parengyodontium album
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29450.33

構造登録者

Soares, A.S.,Brennan, H.M.,Natarajan, R.,McCarthy, L.,Leroy, L. (登録日: 2017-07-18, 公開日: 2017-08-02, 最終更新日: 2024-11-20)

主引用文献

Samara, Y.N.,Brennan, H.M.,McCarthy, L.,Bollard, M.T.,Laspina, D.,Wlodek, J.M.,Campos, S.L.,Natarajan, R.,Gofron, K.,McSweeney, S.,Soares, A.S.,Leroy, L.
Using sound pulses to solve the crystal-harvesting bottleneck.
Acta Crystallogr D Struct Biol, 74:986-999, 2018

PubMed Abstract: Crystal harvesting has proven to be difficult to automate and remains the rate-limiting step for many structure-determination and high-throughput screening projects. This has resulted in crystals being prepared more rapidly than they can be harvested for X-ray data collection. Fourth-generation synchrotrons will support extraordinarily rapid rates of data acquisition, putting further pressure on the crystal-harvesting bottleneck. Here, a simple solution is reported in which crystals can be acoustically harvested from slightly modified MiTeGen In Situ-1 crystallization plates. This technique uses an acoustic pulse to eject each crystal out of its crystallization well, through a short air column and onto a micro-mesh (improving on previous work, which required separately grown crystals to be transferred before harvesting). Crystals can be individually harvested or can be serially combined with a chemical library such as a fragment library.

PubMed: 30289409
DOI: 10.1107/S2059798318011506

実験手法

X-RAY DIFFRACTION (1.71 Å)