5WDE

Crystal structure of the KIFC3 motor domain in complex with ADP

「2H58」から置き換えられました

5WDE の概要

• エントリーDOI: 10.2210/pdb5wde/pdb
• 分子名称: Kinesin-like protein KIFC3, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: kinesin, motor domain, adp, structural genomics, structur al genomics consortium, sgc, structural genomics consortium, motor protein
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cell junction, adherens junction : Q9BVG8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36673.03

構造登録者

Shen, Y.,Tempel, W.,Landry, R.,Arrowsmith, C.H.,Edwards, A.M.,Park, H.,Structural Genomics Consortium (SGC) (登録日: 2017-07-05, 公開日: 2017-08-09, 最終更新日: 2023-10-04)

主引用文献

Park, H.W.,Ma, Z.,Zhu, H.,Jiang, S.,Robinson, R.C.,Endow, S.A.
Structural basis of small molecule ATPase inhibition of a human mitotic kinesin motor protein.
Sci Rep, 7:15121-15121, 2017

PubMed Abstract: Kinesin microtubule motor proteins play essential roles in division, including attaching chromosomes to spindles and crosslinking microtubules for spindle assembly. Human kinesin-14 KIFC1 is unique in that cancer cells with amplified centrosomes are dependent on the motor for viable division because of its ability to cluster centrosomes and form bipolar spindles, but it is not required for division in almost all normal cells. Screens for small molecule inhibitors of KIFC1 have yielded several candidates for further development, but obtaining structural data to determine their sites of binding has been difficult. Here we compare a previously unreported KIFC1 crystal structure with new structures of two closely related kinesin-14 proteins, Ncd and KIFC3, to determine the potential binding site of a known KIFC1 ATPase inhibitor, AZ82. We analyze the previously identified kinesin inhibitor binding sites and identify features of AZ82 that favor binding to one of the sites, the α4/α6 site. This selectivity can be explained by unique structural features of the KIFC1 α4/α6 binding site. These features may help improve the drug-like properties of AZ82 and other specific KIFC1 inhibitors.

PubMed: 29123223
DOI: 10.1038/s41598-017-14754-6

実験手法

X-RAY DIFFRACTION (1.85 Å)