5WCU

Crystal structure of 167 bp nucleosome bound to the globular domain of linker histone H5

5WCU の概要

• エントリーDOI: 10.2210/pdb5wcu/pdb
• 分子名称: Histone H3, Histone H4, Histone H2A, ... (7 entities in total)
• 機能のキーワード: nucleosome core particle, histone fold, chromosome, chromatin, globular domain, histone h5, gh5, 167 bp nucleosome, chromatosome, nucleosome packing, 30 nm chromatin fiber, linker histone h5, linker dna, nucleosome binding protein, protein dna complexes, dna binding, chromatin higher order structure, chromatin folding, chromatin binding protein-dna complex, chromatin binding protein/dna
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• タンパク質・核酸の鎖数: 22
• 化学式量合計: 393467.21

構造登録者

Jiang, J.S.,Zhou, B.R. (登録日: 2017-07-02, 公開日: 2018-10-31, 最終更新日: 2023-10-04)

主引用文献

Zhou, B.R.,Jiang, J.,Ghirlando, R.,Norouzi, D.,Sathish Yadav, K.N.,Feng, H.,Wang, R.,Zhang, P.,Zhurkin, V.,Bai, Y.
Revisit of Reconstituted 30-nm Nucleosome Arrays Reveals an Ensemble of Dynamic Structures.
J. Mol. Biol., 430:3093-3110, 2018

PubMed Abstract: It has long been suggested that chromatin may form a fiber with a diameter of ~30 nm that suppresses transcription. Despite nearly four decades of study, the structural nature of the 30-nm chromatin fiber and conclusive evidence of its existence in vivo remain elusive. The key support for the existence of specific 30-nm chromatin fiber structures is based on the determination of the structures of reconstituted nucleosome arrays using X-ray crystallography and single-particle cryo-electron microscopy coupled with glutaraldehyde chemical cross-linking. Here we report the characterization of these nucleosome arrays in solution using analytical ultracentrifugation, NMR, and small-angle X-ray scattering. We found that the physical properties of these nucleosome arrays in solution are not consistent with formation of just a few discrete structures of nucleosome arrays. In addition, we obtained a crystal of the nucleosome in complex with the globular domain of linker histone H5 that shows a new form of nucleosome packing and suggests a plausible alternative compact conformation for nucleosome arrays. Taken together, our results challenge the key evidence for the existence of a limited number of structures of reconstituted nucleosome arrays in solution by revealing that the reconstituted nucleosome arrays are actually best described as an ensemble of various conformations with a zigzagged arrangement of nucleosomes. Our finding has implications for understanding the structure and function of chromatin in vivo.

PubMed: 29959925
DOI: 10.1016/j.jmb.2018.06.020

実験手法

X-RAY DIFFRACTION (5.53 Å)