5WCO

Matrix Protein (M1) of Infectious Salmon Anaemia Virus

5WCO の概要

• エントリーDOI: 10.2210/pdb5wco/pdb
• 分子名称: NS2 (2 entities in total)
• 機能のキーワード: matrix protein, isav, orthomyxovirus, helical, viral protein
• 由来する生物種: Infectious salmon anemia virus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 72511.18

構造登録者

Zhang, W.,Zheng, W.,Toh, Y.,Betancourt, M.A.,Tu, J.,Fan, Y.,Vakharia, V.,Liu, J.,McNew, J.A.,Jin, M.,Tao, Y.J. (登録日: 2017-07-01, 公開日: 2017-08-16, 最終更新日: 2024-10-16)

主引用文献

Zhang, W.,Zheng, W.,Toh, Y.,Betancourt-Solis, M.A.,Tu, J.,Fan, Y.,Vakharia, V.N.,Liu, J.,McNew, J.A.,Jin, M.,Tao, Y.J.
Crystal structure of an orthomyxovirus matrix protein reveals mechanisms for self-polymerization and membrane association.
Proc. Natl. Acad. Sci. U.S.A., 114:8550-8555, 2017

PubMed Abstract: Many enveloped viruses encode a matrix protein. In the influenza A virus, the matrix protein M1 polymerizes into a rigid protein layer underneath the viral envelope to help enforce the shape and structural integrity of intact viruses. The influenza virus M1 is also known to mediate virus budding as well as the nuclear export of the viral nucleocapsids and their subsequent packaging into nascent viral particles. Despite extensive studies on the influenza A virus M1 (FLUA-M1), only crystal structures of its N-terminal domain are available. Here we report the crystal structure of the full-length M1 from another orthomyxovirus that infects fish, the infectious salmon anemia virus (ISAV). The structure of ISAV-M1 assumes the shape of an elbow, with its N domain closely resembling that of the FLUA-M1. The C domain, which is connected to the N domain through a flexible linker, is made of four α-helices packed as a tight bundle. In the crystal, ISAV-M1 monomers form infinite 2D arrays with a network of interactions involving both the N and C domains. Results from liposome flotation assays indicated that ISAV-M1 binds membrane via electrostatic interactions that are primarily mediated by a positively charged surface loop from the N domain. Cryoelectron tomography reconstruction of intact ISA virions identified a matrix protein layer adjacent to the inner leaflet of the viral membrane. The physical dimensions of the virion-associated matrix layer are consistent with the 2D ISAV-M1 crystal lattice, suggesting that the crystal lattice is a valid model for studying M1-M1, M1-membrane, and M1-RNP interactions in the virion.

PubMed: 28739952
DOI: 10.1073/pnas.1701747114

実験手法

X-RAY DIFFRACTION (2.604 Å)