5WC7

CypA Mutant - I97V S99T C115S

5WC7 の概要

• エントリーDOI: 10.2210/pdb5wc7/pdb
• 分子名称: Peptidyl-prolyl cis-trans isomerase A (2 entities in total)
• 機能のキーワード: proline isomerase, isomerase
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cytoplasm : P62937
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18020.44

構造登録者

Fraser, J.S. (登録日: 2017-06-29, 公開日: 2018-04-18, 最終更新日: 2023-10-04)

主引用文献

Otten, R.,Liu, L.,Kenner, L.R.,Clarkson, M.W.,Mavor, D.,Tawfik, D.S.,Kern, D.,Fraser, J.S.
Rescue of conformational dynamics in enzyme catalysis by directed evolution.
Nat Commun, 9:1314-1314, 2018

PubMed Abstract: Rational design and directed evolution have proved to be successful approaches to increase catalytic efficiencies of both natural and artificial enzymes. Protein dynamics is recognized as important, but due to the inherent flexibility of biological macromolecules it is often difficult to distinguish which conformational changes are directly related to function. Here, we use directed evolution on an impaired mutant of the proline isomerase CypA and identify two second-shell mutations that partially restore its catalytic activity. We show both kinetically, using NMR spectroscopy, and structurally, by room-temperature X-ray crystallography, how local perturbations propagate through a large allosteric network to facilitate conformational dynamics. The increased catalysis selected for in the evolutionary screen is correlated with an accelerated interconversion between the two catalytically essential conformational sub-states, which are both captured in the high-resolution X-ray ensembles. Our data provide a glimpse of an evolutionary trajectory and show how subtle changes can fine-tune enzyme function.

PubMed: 29615624
DOI: 10.1038/s41467-018-03562-9

実験手法

X-RAY DIFFRACTION (1.43 Å)