5WBC

Designed Artificial Cupredoxins - WT

「5K49」から置き換えられました

5WBC の概要

• エントリーDOI: 10.2210/pdb5wbc/pdb
• 分子名称: Streptavidin, [CuII(biot-et-dpea)]2+, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: beta barrel, cupredoxin, copper complex, artificial metalloprotein, biotin binding protein, metal binding protein
• 由来する生物種: Streptomyces avidinii
• 細胞内の位置: Secreted: P22629
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17313.37

構造登録者

Mann, S.I.,Heinisch, T.,Weitz, A.C.,Hendrich, M.R.,Ward, T.R.,Borovik, A.S. (登録日: 2017-06-28, 公開日: 2017-08-16, 最終更新日: 2023-10-04)

主引用文献

Mann, S.I.,Heinisch, T.,Weitz, A.C.,Hendrich, M.P.,Ward, T.R.,Borovik, A.S.
Modular Artificial Cupredoxins.
J. Am. Chem. Soc., 138:9073-9076, 2016

PubMed Abstract: Cupredoxins are electron-transfer proteins that have active sites containing a mononuclear Cu center with an unusual trigonal monopyramidal structure (Type 1 Cu). A single Cu-Scys bond is present within the trigonal plane that is responsible for its unique physical properties. We demonstrate that a cysteine-containing variant of streptavidin (Sav) can serve as a protein host to model the structure and properties of Type 1 Cu sites. A series of artificial Cu proteins are described that rely on Sav and a series of biotinylated synthetic Cu complexes. Optical and EPR measurements highlight the presence of a Cu-Scys bond, and XRD analysis provides structural evidence. We further provide evidence that changes in the linker between the biotin and Cu complex within the synthetic constructs allows for small changes in the placement of Cu centers within Sav that have dramatic effects on the structural and physical properties of the resulting artificial metalloproteins. These findings highlight the utility of the biotin-Sav technology as an approach for simulating active sites of metalloproteins.

PubMed: 27385206
DOI: 10.1021/jacs.6b05428

実験手法

X-RAY DIFFRACTION (1.72 Å)