5WAN

Crystal Structure of a flavoenzyme RutA in the pyrimidine catabolic pathway

5WAN の概要

• エントリーDOI: 10.2210/pdb5wan/pdb
• 分子名称: Pyrimidine monooxygenase RutA, URACIL, SULFATE ION, ... (6 entities in total)
• 機能のキーワード: flavoenzyme, oxidoreductase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45831.34

構造登録者

Zhang, Y.,Mukherjee, T.,Abdelwahed, S.,Begley, T.P.,Ealick, S.E. (登録日: 2017-06-26, 公開日: 2017-07-19, 最終更新日: 2023-10-04)

主引用文献

Mukherjee, T.,Zhang, Y.,Abdelwahed, S.,Ealick, S.E.,Begley, T.P.
Catalysis of a flavoenzyme-mediated amide hydrolysis.
J. Am. Chem. Soc., 132:5550-1, 2010

PubMed Abstract: A new pyrimidine catabolic pathway (the Rut pathway) was recently discovered in Escherichia coli K12. In this pathway, uracil is converted to 3-hydroxypropionate, ammonia, and carbon dioxide. The seven-gene Rut operon is required for this conversion. Here we demonstrate that the flavoenzyme RutA catalyzes the initial uracil ring-opening reaction to give 3-ureidoacrylate. This reaction, while formally a hydrolysis reaction, proceeds by an oxidative mechanism initiated by the addition of a flavin hydroperoxide to the C4 carbonyl. While peroxide-catalyzed amide hydrolysis has chemical precedent, we are not aware of a prior example of analogous chemistry catalyzed by flavin hydroperoxides. This study further illustrates the extraordinary catalytic versatility of the flavin cofactor.

PubMed: 20369853
DOI: 10.1021/ja9107676

実験手法

X-RAY DIFFRACTION (1.798 Å)