5W7G

An envelope of a filamentous hyperthermophilic virus carries lipids in a horseshoe conformation

5W7G の概要

• エントリーDOI: 10.2210/pdb5w7g/pdb
• EMDBエントリー: 8780
• 分子名称: ORF140, ORF132, DNA (253-MER) (3 entities in total)
• 機能のキーワード: afv1, hyperthermophile, filamentous virus, a-form dna, virus
• 由来する生物種: Acidianus filamentous virus 1 (AFV-1); 詳細
• タンパク質・核酸の鎖数: 44
• 化学式量合計: 803345.05

構造登録者

Kasson, P.,DiMaio, F.,Yu, X.,Lucas-Staat, S.,Krupovic, M.,Schouten, S.,Prangishvili, D.,Egelman, E. (登録日: 2017-06-19, 公開日: 2017-07-19, 最終更新日: 2024-03-13)

主引用文献

Egelman, E.H.,Kasson, P.M.,DiMaio, F.,Yu, X.,Lucas-Staat, S.,Krupovic, M.,Schouten, S.,Prangishvili, D.
Model for a novel membrane envelope in a filamentous hyperthermophilic virus.
Elife, 6:-, 2017

PubMed Abstract: Biological membranes create compartments, and are usually formed by lipid bilayers. However, in hyperthermophilic archaea that live optimally at temperatures above 80°C the membranes are monolayers which resemble fused bilayers. Many double-stranded DNA viruses which parasitize such hosts, including the filamentous virus AFV1 of , are enveloped with a lipid-containing membrane. Using cryo-EM, we show that the membrane in AFV1 is a ~2 nm-thick monolayer, approximately half the expected membrane thickness, formed by host membrane-derived lipids which adopt a U-shaped 'horseshoe' conformation. We hypothesize that this unusual viral envelope structure results from the extreme curvature of the viral capsid, as 'horseshoe' lipid conformations favor such curvature and host membrane lipids that permit horseshoe conformations are selectively recruited into the viral envelope. The unusual envelope found in AFV1 also has many implications for biotechnology, since this membrane can survive the most aggressive conditions involving extremes of temperature and pH.

PubMed: 28639939
DOI: 10.7554/eLife.26268

実験手法

ELECTRON MICROSCOPY (4.5 Å)