5W46

Structure of S65D Phosphomimetic Ubiquitin Refined at 1.2 Angstroms Resolution

5W46 の概要

• エントリーDOI: 10.2210/pdb5w46/pdb
• 分子名称: Polyubiquitin-B, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: ubiquitin, phosphomimic, signaling protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 17233.99

構造登録者

Kazansky, Y.,Singh, R.K.,Fushman, D. (登録日: 2017-06-09, 公開日: 2017-07-05, 最終更新日: 2023-10-04)

主引用文献

Kazansky, Y.,Lai, M.Y.,Singh, R.K.,Fushman, D.
Impact of different ionization states of phosphorylated Serine-65 on ubiquitin structure and interactions.
Sci Rep, 8:2651-2651, 2018

PubMed Abstract: The covalent attachment of ubiquitin (Ub) or Ub chains to cellular proteins is a versatile post-translational modification involved in a variety of eukaryotic cellular events. Recently, the post-translational modification of Ub itself by phosphorylation has emerged as an important component of the Ub-signaling system. Specifically, Ub phosphorylation at serine-65 was shown to activate parkin-mediated mitochondrial quality control. However, the impact of phosphorylation on Ub structure and interactions is poorly understood. Here we investigate the recently reported structural changes in Ub upon serine-65 phosphorylation, namely, the equilibrium between a native-like and a novel, alternate conformer of phosphorylated Ub (pUb). We show that this equilibrium is pH-dependent, and the two pUb conformers are linked to the different charge states of the phosphate group. We examined pUb binding to a known Ub-receptor and found that the alternate conformer is binding incompetent. Furthermore, serine-65 phosphorylation affects the conformational equilibrium of K48-linked Ub dimers. Lastly, our crystal structure of S65D Ub and NMR data indicate that phosphomimetic mutations do not adequately reproduce the salient features of pUb. Our results suggest that the pH-dependence of the conformations and binding properties of phosphorylated Ub and polyUb could provide an additional level of modulation in Ub-mediated signaling.

PubMed: 29422536
DOI: 10.1038/s41598-018-20860-w

実験手法

X-RAY DIFFRACTION (1.18 Å)