5W40

Crystal structure of PopP2 F318S in complex with IP6 and AcCoA

5W40 の概要

• エントリーDOI: 10.2210/pdb5w40/pdb
• 関連するPDBエントリー: 5W3T 5W3X 5W3Y
• 分子名称: PopP2 protein, INOSITOL HEXAKISPHOSPHATE, COENZYME A, ... (4 entities in total)
• 機能のキーワード: popp2, ip6, accoa, acetyltransferase, yopj, effector, transcription
• 由来する生物種: Ralstonia solanacearum
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 160432.68

構造登録者

Zhang, Z.M.,Gao, L.,Song, J. (登録日: 2017-06-08, 公開日: 2017-08-09, 最終更新日: 2024-10-16)

主引用文献

Zhang, Z.M.,Ma, K.W.,Gao, L.,Hu, Z.,Schwizer, S.,Ma, W.,Song, J.
Mechanism of host substrate acetylation by a YopJ family effector.
Nat Plants, 3:17115-17115, 2017

PubMed Abstract: The Yersinia outer protein J (YopJ) family of bacterial effectors depends on a novel acetyltransferase domain to acetylate signalling proteins from plant and animal hosts. However, the underlying mechanism is unclear. Here, we report the crystal structures of PopP2, a YopJ effector produced by the plant pathogen Ralstonia solanacearum, in complex with inositol hexaphosphate (InsP), acetyl-coenzyme A (AcCoA) and/or substrate Resistance to Ralstonia solanacearum 1 (RRS1-R). PopP2 recognizes the WRKYGQK motif of RRS1-R to position a targeted lysine in the active site for acetylation. Importantly, the PopP2-RRS1-R association is allosterically regulated by InsP binding, suggesting a previously unidentified role of the eukaryote-specific cofactor in substrate interaction. Furthermore, we provide evidence for the reaction intermediate of PopP2-mediated acetylation, an acetyl-cysteine covalent adduct, lending direct support to the 'ping-pong'-like catalytic mechanism proposed for YopJ effectors. Our study provides critical mechanistic insights into the virulence activity of YopJ class of acetyltransferases.

PubMed: 28737762
DOI: 10.1038/nplants.2017.115

実験手法

X-RAY DIFFRACTION (2.53 Å)