5W3C

Crystal structure of mutant CJ YCEI protein (CJ-N182C) with selenocysteine guest structure

5W3C の概要

• エントリーDOI: 10.2210/pdb5w3c/pdb
• 関連するPDBエントリー: 5W17 5W37
• 分子名称: Polyisoprenoid-binding protein, EICOSANE, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: nanotechnology, nanoporous, unknown function
• 由来する生物種: Campylobacter jejuni
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20831.56

構造登録者

Huber, T.R.,Snow, C.D. (登録日: 2017-06-07, 公開日: 2018-01-03, 最終更新日: 2024-11-06)

主引用文献

Huber, T.R.,McPherson, E.C.,Keating, C.E.,Snow, C.D.
Installing Guest Molecules at Specific Sites within Scaffold Protein Crystals.
Bioconjug. Chem., 29:17-22, 2018

PubMed Abstract: Protein crystals are porous self-assembling materials that can be rapidly evolved by mutagenesis. We aimed to develop scaffold assisted crystallography techniques in an engineered protein crystal with large pores (>13 nm). Guest molecules were installed via a single covalent bond to attempt to reduce the conformational freedom and achieve high-occupancy structures. We used four different conjugation strategies to attach guest molecules to three different cysteine sites within pre-existing protein crystals. In all but one case, the presence of the adduct was obvious in the electron density. Structure determination of larger guest molecules may be feasible due to the large pores of the engineered scaffold crystals.

PubMed: 29232505
DOI: 10.1021/acs.bioconjchem.7b00668

実験手法

X-RAY DIFFRACTION (2.87 Å)