5W39

Crystal structure of mutant CJ YCEI protein (CJ-N182C) with monobromobimane guest structure

Summary for 5W39

• Entry DOI: 10.2210/pdb5w39/pdb
• Related: 5W17 5W37
• Descriptor: Polyisoprenoid-binding protein, EICOSANE, SULFATE ION, ... (5 entities in total)
• Functional Keywords: nanotechnology, nanoporous, unknown function
• Biological source: Campylobacter jejuni
• Total number of polymer chains: 1
• Total formula weight: 20934.62

Authors

Huber, T.R.,Snow, C.D. (deposition date: 2017-06-07, release date: 2018-01-03, Last modification date: 2023-10-04)

Primary citation

Huber, T.R.,McPherson, E.C.,Keating, C.E.,Snow, C.D.
Installing Guest Molecules at Specific Sites within Scaffold Protein Crystals.
Bioconjug. Chem., 29:17-22, 2018

PubMed Abstract: Protein crystals are porous self-assembling materials that can be rapidly evolved by mutagenesis. We aimed to develop scaffold assisted crystallography techniques in an engineered protein crystal with large pores (>13 nm). Guest molecules were installed via a single covalent bond to attempt to reduce the conformational freedom and achieve high-occupancy structures. We used four different conjugation strategies to attach guest molecules to three different cysteine sites within pre-existing protein crystals. In all but one case, the presence of the adduct was obvious in the electron density. Structure determination of larger guest molecules may be feasible due to the large pores of the engineered scaffold crystals.

PubMed: 29232505
DOI: 10.1021/acs.bioconjchem.7b00668

Experimental method

X-RAY DIFFRACTION (2.48 Å)