5VZM

Solution NMR structure of human Rev1 (932-1039) in complex with ubiquitin

5VZM の概要

• エントリーDOI: 10.2210/pdb5vzm/pdb
• NMR情報: BMRB: 30300
• 分子名称: Ubiquitin, DNA repair protein REV1 (2 entities in total)
• 機能のキーワード: translesion dna synthesis, dna repair, dna replication, rev1, ubiquitin, pcna, protein binding
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 20562.18

構造登録者

Cui, G.,Botuyan, M.V.,Mer, G. (登録日: 2017-05-29, 公開日: 2018-05-23, 最終更新日: 2024-11-13)

主引用文献

Cui, G.,Botuyan, M.V.,Mer, G.
Structural Basis for the Interaction of Mutasome Assembly Factor REV1 with Ubiquitin.
J. Mol. Biol., 430:2042-2050, 2018

PubMed Abstract: REV1 is an evolutionarily conserved translesion synthesis (TLS) DNA polymerase and an assembly factor key for the recruitment of other TLS polymerases to DNA damage sites. REV1-mediated recognition of ubiquitin in the proliferative cell nuclear antigen is thought to be the trigger for TLS activation. Here we report the solution NMR structure of a 108-residue fragment of human REV1 encompassing the two putative ubiquitin-binding motifs UBM1 and UBM2 in complex with ubiquitin. While in mammals UBM1 and UBM2 are both required for optimal association of REV1 with replication factories after DNA damage, we show that only REV1 UBM2 binds ubiquitin. Structure-guided mutagenesis in Saccharomyces cerevisiae further highlights the importance of UBM2 for REV1-mediated mutagenesis and DNA damage tolerance.

PubMed: 29778604
DOI: 10.1016/j.jmb.2018.05.017

実験手法

SOLUTION NMR