5VY3

Thermoplasma acidophilum 20S Proteasome using 200keV with stage position

5VY3 の概要

• エントリーDOI: 10.2210/pdb5vy3/pdb
• EMDBエントリー: 8741 8742
• 分子名称: Proteasome subunit alpha, Proteasome subunit beta (2 entities in total)
• 機能のキーワード: proteasome, hydrolase
• 由来する生物種: Thermoplasma acidophilum; 詳細
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 658995.81

構造登録者

Herzik Jr., M.A.,Wu, M.,Lander, G.C. (登録日: 2017-05-24, 公開日: 2017-06-14, 最終更新日: 2024-03-13)

主引用文献

Herzik, M.A.,Wu, M.,Lander, G.C.
Achieving better-than-3- angstrom resolution by single-particle cryo-EM at 200 keV.
Nat. Methods, 14:1075-1078, 2017

PubMed Abstract: Nearly all single-particle cryo-EM structures resolved to better than 4-Å resolution have been determined using 300-keV transmission electron microscopes (TEMs). We demonstrate that it is possible to obtain reconstructions of macromolecular complexes of different sizes to better than 3-Å resolution using a 200-keV TEM. These structures are of sufficient quality to unambiguously assign amino acid rotameric conformations and identify ordered water molecules.

PubMed: 28991891
DOI: 10.1038/nmeth.4461

実験手法

ELECTRON MICROSCOPY (3.1 Å)