5VVJ

Cas1-Cas2 bound to half-site intermediate

5VVJ の概要

• エントリーDOI: 10.2210/pdb5vvj/pdb
• 分子名称: CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, DNA (28-MER), ... (4 entities in total)
• 機能のキーワード: complex, dna, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Escherichia coli (strain K12); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 197328.86

構造登録者

Wright, A.V.,Knott, G.J.,Doxzen, K.W.,Doudna, J.A. (登録日: 2017-05-19, 公開日: 2017-08-02, 最終更新日: 2023-10-04)

主引用文献

Wright, A.V.,Liu, J.J.,Knott, G.J.,Doxzen, K.W.,Nogales, E.,Doudna, J.A.
Structures of the CRISPR genome integration complex.
Science, 357:1113-1118, 2017

PubMed Abstract: CRISPR-Cas systems depend on the Cas1-Cas2 integrase to capture and integrate short foreign DNA fragments into the CRISPR locus, enabling adaptation to new viruses. We present crystal structures of Cas1-Cas2 bound to both donor and target DNA in intermediate and product integration complexes, as well as a cryo-electron microscopy structure of the full CRISPR locus integration complex, including the accessory protein IHF (integration host factor). The structures show unexpectedly that indirect sequence recognition dictates integration site selection by favoring deformation of the repeat and the flanking sequences. IHF binding bends the DNA sharply, bringing an upstream recognition motif into contact with Cas1 to increase both the specificity and efficiency of integration. These results explain how the Cas1-Cas2 CRISPR integrase recognizes a sequence-dependent DNA structure to ensure site-selective CRISPR array expansion during the initial step of bacterial adaptive immunity.

PubMed: 28729350
DOI: 10.1126/science.aao0679

実験手法

X-RAY DIFFRACTION (3.89 Å)