5VL9

Crystal structure of EilR in complex with eilO DNA element

5VL9 の概要

• エントリーDOI: 10.2210/pdb5vl9/pdb
• 関連するPDBエントリー: 5VLG 5VLM
• 分子名称: Regulatory protein TetR, DNA (5'-D(*TP*AP*TP*GP*TP*CP*CP*AP*AP*CP*TP*TP*TP*C)-3'), DNA (5'-D(*GP*AP*AP*AP*GP*TP*TP*GP*GP*AP*CP*AP*TP*A)-3'), ... (5 entities in total)
• 機能のキーワード: transcription factor, protein-dna complex, transcription-dna complex, transcription/dna
• 由来する生物種: Enterobacter lignolyticus; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 106520.57

構造登録者

Pereira, J.H.,Ruegg, T.L.,Chen, J.,Novichkov, P.,DeGiovani, A.,Tomaleri, G.P.,Singer, S.,Simmons, B.,Thelen, M.,Adams, P.D. (登録日: 2017-04-25, 公開日: 2018-06-27, 最終更新日: 2022-03-16)

主引用文献

Ruegg, T.L.,Pereira, J.H.,Chen, J.C.,DeGiovanni, A.,Novichkov, P.,Mutalik, V.K.,Tomaleri, G.P.,Singer, S.W.,Hillson, N.J.,Simmons, B.A.,Adams, P.D.,Thelen, M.P.
Jungle Express is a versatile repressor system for tight transcriptional control.
Nat Commun, 9:3617-3617, 2018

PubMed Abstract: Tightly regulated promoters are essential for numerous biological applications, where strong inducibility, portability, and scalability are desirable. Current systems are often incompatible with large-scale fermentations due to high inducer costs and strict media requirements. Here, we describe the bottom-up engineering of 'Jungle Express', an expression system that enables efficient gene regulation in diverse proteobacteria. This system is guided by EilR, a multidrug-binding repressor with high affinity to its optimized operator and cationic dyes that act as powerful inducers at negligible costs. In E. coli, the engineered promoters exhibit minimal basal transcription and are inducible over four orders of magnitude by 1 µM crystal violet, reaching expression levels exceeding those of the strongest current bacterial systems. Further, we provide molecular insights into specific interactions of EilR with its operator and with two inducers. The versatility of Jungle Express opens the way for tightly controlled and efficient gene expression that is not restricted to host organism, substrate, or scale.

PubMed: 30190458
DOI: 10.1038/s41467-018-05857-3

実験手法

X-RAY DIFFRACTION (2.16 Å)