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5VJT

De Novo Photosynthetic Reaction Center Protein Equipped with Heme B and Zn(II) cations

5VJT の概要
エントリーDOI10.2210/pdb5vjt/pdb
関連するPDBエントリー5VJS 5VJU
分子名称Reaction Center Maquette, ZINC ION, PROTOPORPHYRIN IX CONTAINING FE, ... (5 entities in total)
機能のキーワードmaquette, protein design, charge separation, artificial photosynthesis, de novo protein
由来する生物種synthetic construct
タンパク質・核酸の鎖数1
化学式量合計23384.09
構造登録者
Ennist, N.M.,Stayrook, S.E.,Dutton, P.L.,Moser, C.C. (登録日: 2017-04-19, 公開日: 2018-04-25, 最終更新日: 2024-05-22)
主引用文献Ennist, N.M.,Zhao, Z.,Stayrook, S.E.,Discher, B.M.,Dutton, P.L.,Moser, C.C.
De novo protein design of photochemical reaction centers.
Nat Commun, 13:4937-4937, 2022
Cited by
PubMed Abstract: Natural photosynthetic protein complexes capture sunlight to power the energetic catalysis that supports life on Earth. Yet these natural protein structures carry an evolutionary legacy of complexity and fragility that encumbers protein reengineering efforts and obfuscates the underlying design rules for light-driven charge separation. De novo development of a simplified photosynthetic reaction center protein can clarify practical engineering principles needed to build new enzymes for efficient solar-to-fuel energy conversion. Here, we report the rational design, X-ray crystal structure, and electron transfer activity of a multi-cofactor protein that incorporates essential elements of photosynthetic reaction centers. This highly stable, modular artificial protein framework can be reconstituted in vitro with interchangeable redox centers for nanometer-scale photochemical charge separation. Transient absorption spectroscopy demonstrates Photosystem II-like tyrosine and metal cluster oxidation, and we measure charge separation lifetimes exceeding 100 ms, ideal for light-activated catalysis. This de novo-designed reaction center builds upon engineering guidelines established for charge separation in earlier synthetic photochemical triads and modified natural proteins, and it shows how synthetic biology may lead to a new generation of genetically encoded, light-powered catalysts for solar fuel production.
PubMed: 35999239
DOI: 10.1038/s41467-022-32710-5
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (1.45 Å)
構造検証レポート
Validation report summary of 5vjt
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-11-13に公開中

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