5VJB

Guanidine-II riboswitch P2 hairpin dimer with 5-bromoU substitution from Pseudomonas aeruginosa

5VJB の概要

• エントリーDOI: 10.2210/pdb5vjb/pdb
• 関連するPDBエントリー: 5VJ9
• 分子名称: RNA (5'-R(*GP*CP*GP*GP*GP*GP*AP*CP*GP*AP*CP*CP*CP*(5BU)P*GP*C)-3'), GUANIDINE, SPERMINE, ... (5 entities in total)
• 機能のキーワード: guanidine, riboswitch, kissing loop, hairpin, rna
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 22054.13

構造登録者

Reiss, C.W.,Strobel, S.A. (登録日: 2017-04-19, 公開日: 2017-06-28, 最終更新日: 2024-03-13)

主引用文献

Reiss, C.W.,Strobel, S.A.
Structural basis for ligand binding to the guanidine-II riboswitch.
RNA, 23:1338-1343, 2017

PubMed Abstract: The guanidine-II riboswitch, also known as , is a conserved mRNA element with more than 800 examples in bacteria. It consists of two stem-loops capped by identical, conserved tetraloops that are separated by a linker region of variable length and sequence. Like the guanidine-I riboswitch, it controls the expression of guanidine carboxylases and SugE-like genes. The guanidine-II riboswitch specifically binds free guanidinium cations and functions as a translationally controlled on-switch. Here we report the structure of a P2 stem-loop from the guanidine-II riboswitch aptamer bound to guanidine at 1.57 Å resolution. The hairpins dimerize via the conserved tetraloop, which also contains the binding pocket. Two guanidinium molecules bind near the dimerization interface, one in each tetraloop. The guanidinium cation is engaged in extensive hydrogen bonding to the RNA. Contacts include the Hoogsteen face of a guanine base and three nonbridging phosphate oxygens. Cation-π interactions and ionic interactions also stabilize ligand binding. The guanidine-II riboswitch utilizes the same recognition strategies as the guanidine-I riboswitch while adopting an entirely different and much smaller RNA fold.

PubMed: 28600356
DOI: 10.1261/rna.061804.117

実験手法

X-RAY DIFFRACTION (2.1 Å)