5V8E

Structure of Bacillus cereus PatB1

5V8E の概要

• エントリーDOI: 10.2210/pdb5v8e/pdb
• 関連するPDBエントリー: 5V8D
• 分子名称: Bacillus cereus PatB1, CITRIC ACID, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: acetyltransferase, cell wall, sgnh hydrolase-like, transferase, unknown function
• 由来する生物種: Bacillus cereus (strain ATCC 10987 / NRS 248)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 72529.81

構造登録者

Sychantha, D.,Little, D.J.,Chapman, R.N.,Boons, G.J.,Robinson, H.,Howell, P.L.,Clarke, A.J. (登録日: 2017-03-21, 公開日: 2017-10-18, 最終更新日: 2024-10-23)

主引用文献

Sychantha, D.,Little, D.J.,Chapman, R.N.,Boons, G.J.,Robinson, H.,Howell, P.L.,Clarke, A.J.
PatB1 is an O-acetyltransferase that decorates secondary cell wall polysaccharides.
Nat. Chem. Biol., 14:79-85, 2018

PubMed Abstract: O-Acetylation of the secondary cell wall polysaccharides (SCWP) of the Bacillus cereus group of pathogens, which includes Bacillus anthracis, is essential for the proper attachment of surface-layer (S-layer) proteins to their cell walls. Using a variety of pseudosubstrates and a chemically synthesized analog of SCWP, we report here the identification of PatB1 as a SCWP O-acetyltransferase in Bacillus cereus. Additionally, we report the crystal structure of PatB1, which provides detailed insights into the mechanism of this enzyme and defines a novel subfamily of the SGNH family of esterases and lipases. We propose a model for the O-acetylation of SCWP requiring the translocation of acetyl groups from a cytoplasmic source across the plasma membrane by PatA1 and PatA2 for their transfer to SCWP by PatB1.

PubMed: 29083419
DOI: 10.1038/nchembio.2509

実験手法

X-RAY DIFFRACTION (2.2 Å)