5V3I

Crystal structure of the VS ribozyme - wild-type C634

5V3I の概要

• エントリーDOI: 10.2210/pdb5v3i/pdb
• 分子名称: VS Ribozyme RNA (2 entities in total)
• 機能のキーワード: shifted substrate, rna
• 由来する生物種: Neurospora
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 60046.69

構造登録者

DasGupta, S.,Suslov, N.B.,Piccirilli, J.A. (登録日: 2017-03-07, 公開日: 2017-07-05, 最終更新日: 2023-10-04)

主引用文献

DasGupta, S.,Suslov, N.B.,Piccirilli, J.A.
Structural Basis for Substrate Helix Remodeling and Cleavage Loop Activation in the Varkud Satellite Ribozyme.
J. Am. Chem. Soc., 139:9591-9597, 2017

PubMed Abstract: The Varkud satellite (VS) ribozyme catalyzes site-specific RNA cleavage and ligation reactions. Recognition of the substrate involves a kissing loop interaction between the substrate and the catalytic domain of the ribozyme, resulting in a rearrangement of the substrate helix register into a so-called "shifted" conformation that is critical for substrate binding and activation. We report a 3.3 Å crystal structure of the complete ribozyme that reveals the active, shifted conformation of the substrate, docked into the catalytic domain of the ribozyme. Comparison to previous NMR structures of isolated, inactive substrates provides a physical description of substrate remodeling, and implicates roles for tertiary interactions in catalytic activation of the cleavage loop. Similarities to the hairpin ribozyme cleavage loop activation suggest general strategies to enhance fidelity in RNA folding and ribozyme cleavage.

PubMed: 28625058
DOI: 10.1021/jacs.7b03655

実験手法

X-RAY DIFFRACTION (3.293 Å)