5V2C

RE-REFINEMENT OF CRYSTAL STRUCTURE OF PHOTOSYSTEM II COMPLEX

5V2C の概要

• エントリーDOI: 10.2210/pdb5v2c/pdb
• 分子名称: Photosystem II protein D1, Photosystem II reaction center protein K, Photosystem II reaction center protein L, ... (46 entities in total)
• 機能のキーワード: electron transport, photosynthesis
• 由来する生物種: Thermosynechococcus vulcanus; 詳細
• タンパク質・核酸の鎖数: 38
• 化学式量合計: 767989.79

構造登録者

Wang, J.,Wiwczar, J.M.,Brudvig, G.W. (登録日: 2017-03-03, 公開日: 2017-08-30, 最終更新日: 2023-10-04)

主引用文献

Wiwczar, J.M.,LaFountain, A.M.,Wang, J.,Frank, H.A.,Brudvig, G.W.
Chlorophyll a with a farnesyl tail in thermophilic cyanobacteria.
Photosyn. Res., 134:175-182, 2017

PubMed Abstract: Photosystem II (PSII) of oxygenic photosynthetic organisms normally contains exclusively chlorophyll a (Chl a) as its major light-harvesting pigment. Chl a canonically consists of the chlorin headgroup with a 20-carbon, 4-isoprene unit, phytyl tail. We have examined the 1.9 Å crystal structure of PSII from thermophilic cyanobacteria reported by Shen and coworkers in 2012 (PDB accession of 3ARC/3WU2). A newly refined electron density map from this structure, presented here, reveals that some assignments of the cofactors may be different from those modeled in the 3ARC/3WU2 structure, including a specific Chl a that appears to have a truncated tail by one isoprene unit. We provide experimental evidence using high-performance liquid chromatography and mass spectrometry for a small population of Chl a esterified to a 15-carbon farnesyl tail in PSII of thermophilic cyanobacteria.

PubMed: 28741056
DOI: 10.1007/s11120-017-0425-4

実験手法

X-RAY DIFFRACTION (1.9 Å)