5UQD

DPY-21 in complex with Fe(II) and alpha-Ketoglutarate

5UQD の概要

• エントリーDOI: 10.2210/pdb5uqd/pdb
• 分子名称: DumPY: shorter than wild-type, 2-OXOGLUTARIC ACID, FE (II) ION, ... (5 entities in total)
• 機能のキーワード: oxidoreductase, jmjc, h4k20me2 demethylase
• 由来する生物種: Caenorhabditis elegans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 51074.01

構造登録者

Brejc, K.,Bian, Q.,Uzawa, S.,Wheeler, B.S.,Anderson, E.C.,King, D.S.,Kranzusch, P.J.,Preston, C.G.,Meyer, B.J. (登録日: 2017-02-07, 公開日: 2017-09-13, 最終更新日: 2024-03-06)

主引用文献

Brejc, K.,Bian, Q.,Uzawa, S.,Wheeler, B.S.,Anderson, E.C.,King, D.S.,Kranzusch, P.J.,Preston, C.G.,Meyer, B.J.
Dynamic Control of X Chromosome Conformation and Repression by a Histone H4K20 Demethylase.
Cell, 171:85-102.e23, 2017

PubMed Abstract: Chromatin modification and higher-order chromosome structure play key roles in gene regulation, but their functional interplay in controlling gene expression is elusive. We have discovered the machinery and mechanism underlying the dynamic enrichment of histone modification H4K20me1 on hermaphrodite X chromosomes during C. elegans dosage compensation and demonstrated H4K20me1's pivotal role in regulating higher-order chromosome structure and X-chromosome-wide gene expression. The structure and the activity of the dosage compensation complex (DCC) subunit DPY-21 define a Jumonji demethylase subfamily that converts H4K20me2 to H4K20me1 in worms and mammals. Selective inactivation of demethylase activity eliminates H4K20me1 enrichment in somatic cells, elevates X-linked gene expression, reduces X chromosome compaction, and disrupts X chromosome conformation by diminishing the formation of topologically associating domains (TADs). Unexpectedly, DPY-21 also associates with autosomes of germ cells in a DCC-independent manner to enrich H4K20me1 and trigger chromosome compaction. Our findings demonstrate the direct link between chromatin modification and higher-order chromosome structure in long-range regulation of gene expression.

PubMed: 28867287
DOI: 10.1016/j.cell.2017.07.041

実験手法

X-RAY DIFFRACTION (1.798 Å)