5UNK

NMR structure of the RED subdomain of the Sleeping Beauty transposase

5UNK の概要

• エントリーDOI: 10.2210/pdb5unk/pdb
• NMR情報: BMRB: 30239
• 分子名称: Sleeping Beauty transposase (1 entity in total)
• 機能のキーワード: helical, helix-turn-helix, dna-binding, transposase, dna binding protein
• 由来する生物種: Oncorhynchus mykiss (Rainbow trout)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7122.41

構造登録者

Konnova, T.A.,Singer, C.M.,Nesmelova, I.V. (登録日: 2017-01-31, 公開日: 2017-06-07, 最終更新日: 2024-05-15)

主引用文献

Konnova, T.A.,Singer, C.M.,Nesmelova, I.V.
NMR solution structure of the RED subdomain of the Sleeping Beauty transposase.
Protein Sci., 26:1171-1181, 2017

PubMed Abstract: DNA transposons can be employed for stable gene transfer in vertebrates. The Sleeping Beauty (SB) DNA transposon has been recently adapted for human application and is being evaluated in clinical trials, however its molecular mechanism is not clear. SB transposition is catalyzed by the transposase enzyme, which is a multi-domain protein containing the catalytic and the DNA-binding domains. The DNA-binding domain of the SB transposase contains two structurally independent subdomains, PAI and RED. Recently, the structures of the catalytic domain and the PAI subdomain have been determined, however no structural information on the RED subdomain and its interactions with DNA has been available. Here, we used NMR spectroscopy to determine the solution structure of the RED subdomain and characterize its interactions with the transposon DNA.

PubMed: 28345263
DOI: 10.1002/pro.3167

実験手法

SOLUTION NMR