5UKZ

NMR Solution structure of chemically synthesized antilisterial Pediocin PA-1 M31L analog.

Summary for 5UKZ

• Entry DOI: 10.2210/pdb5ukz/pdb
• NMR Information: BMRB: 30238
• Descriptor: Bacteriocin pediocin PA-1 M31L (1 entity in total)
• Functional Keywords: antilisterial, pediocin pa-1, pediococcus acidilactici ul5, bacteriocin, chemical synthesis, antimicrobial protein
• Biological source: Pediococcus acidilactici
• Total number of polymer chains: 1
• Total formula weight: 4617.17

Authors

Bedard, F.,Hammami, R.,Zirah, S.,Rebuffat, S.,Fliss, I.,Biron, E. (deposition date: 2017-01-23, release date: 2018-06-27)

Primary citation

Bedard, F.,Hammami, R.,Zirah, S.,Rebuffat, S.,Fliss, I.,Biron, E.
Synthesis, antimicrobial activity and conformational analysis of the class IIa bacteriocin pediocin PA-1 and analogs thereof.
Sci Rep, 8:9029-9029, 2018

PubMed Abstract: The antimicrobial peptide pediocin PA-1 is a class IIa bacteriocin that inhibits several clinically relevant pathogens including Listeria spp. Here we report the synthesis and characterization of whole pediocin PA-1 and novel analogs thereof using a combination of solid- and solution-phase strategies to overcome difficulties due to instability and undesired reactions. Pediocin PA-1 thus synthesized was a potent inhibitor of Listeria monocytogenes (MIC = 6.8 nM), similar to the bacteriocin produced naturally by Pediococcus acidilactici. Of particular interest is that linear analogs lacking both of the disulfide bridges characterizing pediocin PA-1 were as potent. One linear analog was also a strong inhibitor of Clostridium perfringens, another important food-borne pathogen. These results are discussed in light of conformational information derived from circular dichroism, solution NMR spectroscopy and structure-activity relationship studies.

PubMed: 29899567
DOI: 10.1038/s41598-018-27225-3

Experimental method

SOLUTION NMR