5UK3

Crystal structure of cyanase from T. urticae

5UK3 の概要

• エントリーDOI: 10.2210/pdb5uk3/pdb
• 分子名称: Uncharacterized protein (2 entities in total)
• 機能のキーワード: tetranychus urticae, cyanase, agricultural pest, two-spotted spidermite, spider mite, lyase
• 由来する生物種: Tetranychus urticae (Two-spotted spider mite)
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 202410.80

構造登録者

Schlachter, C.R.,Chruszcz, M. (登録日: 2017-01-19, 公開日: 2017-06-21, 最終更新日: 2023-10-04)

主引用文献

Schlachter, C.R.,Klapper, V.,Wybouw, N.,Radford, T.,Van Leeuwen, T.,Grbic, M.,Chruszcz, M.
Structural Characterization of a Eukaryotic Cyanase from Tetranychus urticae.
J. Agric. Food Chem., 65:5453-5462, 2017

PubMed Abstract: The two-spotted spider mite Tetranychus urticae is a polyphagous agricultural pest and poses a high risk to global crop production as it is rapidly developing pesticide resistance. Genomic and transcriptomic analysis has revealed the presence of a remarkable cyanase gene in T. urticae and related mite species within the Acariformes lineage. Cyanase catalyzes the detoxification of cyanate and is potentially an attractive protein target for the development of new acaricides. Phylogenetic analysis indicates that within the Acariformes, the cyanase gene originates from a single horizontal gene transfer event, which precedes subsequent speciation. Our structural studies presented here compare and contrast prokaryotic cyanases to T. urticae cyanase, which all form homodecamers and have conserved active site residues, but display different surface areas between homodimers in the overall decameric structure.

PubMed: 28613863
DOI: 10.1021/acs.jafc.7b01333

実験手法

X-RAY DIFFRACTION (2.8 Å)