5UJX

Crystal structure of DHFR in 20% Isopropanol

5UJX の概要

• エントリーDOI: 10.2210/pdb5ujx/pdb
• 分子名称: Dihydrofolate reductase, FOLIC ACID, ISOPROPYL ALCOHOL, ... (6 entities in total)
• 機能のキーワード: dynamics, oxidoreductase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37165.43

構造登録者

Cuneo, M.J.,Agarwal, P.K. (登録日: 2017-01-19, 公開日: 2017-12-27, 最終更新日: 2023-10-04)

主引用文献

Duff Jr., M.R.,Borreguero, J.M.,Cuneo, M.J.,Ramanathan, A.,He, J.,Kamath, G.,Chennubhotla, S.C.,Meilleur, F.,Howell, E.E.,Herwig, K.W.,Myles, D.A.A.,Agarwal, P.K.
Modulating Enzyme Activity by Altering Protein Dynamics with Solvent.
Biochemistry, 57:4263-4275, 2018

PubMed Abstract: Optimal enzyme activity depends on a number of factors, including structure and dynamics. The role of enzyme structure is well recognized; however, the linkage between protein dynamics and enzyme activity has given rise to a contentious debate. We have developed an approach that uses an aqueous mixture of organic solvent to control the functionally relevant enzyme dynamics (without changing the structure), which in turn modulates the enzyme activity. Using this approach, we predicted that the hydride transfer reaction catalyzed by the enzyme dihydrofolate reductase (DHFR) from Escherichia coli in aqueous mixtures of isopropanol (IPA) with water will decrease by ∼3 fold at 20% (v/v) IPA concentration. Stopped-flow kinetic measurements find that the pH-independent k rate decreases by 2.2 fold. X-ray crystallographic enzyme structures show no noticeable differences, while computational studies indicate that the transition state and electrostatic effects were identical for water and mixed solvent conditions; quasi-elastic neutron scattering studies show that the dynamical enzyme motions are suppressed. Our approach provides a unique avenue to modulating enzyme activity through changes in enzyme dynamics. Further it provides vital insights that show the altered motions of DHFR cause significant changes in the enzyme's ability to access its functionally relevant conformational substates, explaining the decreased k rate. This approach has important implications for obtaining fundamental insights into the role of rate-limiting dynamics in catalysis and as well as for enzyme engineering.

PubMed: 29901984
DOI: 10.1021/acs.biochem.8b00424

実験手法

X-RAY DIFFRACTION (1.8 Å)