5UJE

SbnI with C-terminal truncation from Staphylococcus aureus

5UJE の概要

• エントリーDOI: 10.2210/pdb5uje/pdb
• 関連するPDBエントリー: 5UJD
• 分子名称: SbnI protein, GLYCEROL (3 entities in total)
• 機能のキーワード: staphyloferrin b, heme, regulator, siderophore, gene regulation
• 由来する生物種: Staphylococcus aureus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28384.61

構造登録者

Murphy, M.E.P.,Verstraete, M.M. (登録日: 2017-01-17, 公開日: 2018-01-24, 最終更新日: 2024-10-23)

主引用文献

Verstraete, M.M.,Perez-Borrajero, C.,Brown, K.L.,Heinrichs, D.E.,Murphy, M.E.P.
SbnI is a free serine kinase that generates O -phospho-l-serine for staphyloferrin B biosynthesis in Staphylococcus aureus .
J.Biol.Chem., 293:6147-6160, 2018

PubMed Abstract: Staphyloferrin B (SB) is an iron-chelating siderophore produced by in invasive infections. Proteins for SB biosynthesis and export are encoded by the gene cluster, in which SbnI, a member of the ParB/Srx superfamily, acts as a heme-dependent transcriptional regulator of the locus. However, no structural or functional information about SbnI is available. Here, a crystal structure of SbnI revealed striking structural similarity to an ADP-dependent free serine kinase, SerK, from the archaea We found that features of the active sites are conserved, and biochemical assays and P NMR and HPLC analyses indicated that SbnI is also a free serine kinase but uses ATP rather than ADP as phosphate donor to generate the SB precursor -phospho-l-serine (OPS). SbnI consists of two domains, and elevated -factors in domain II were consistent with the open-close reaction mechanism previously reported for SerK. Mutagenesis of Glu and Asp in SbnI disclosed that they are required for kinase activity. The only known OPS source in bacteria is through the phosphoserine aminotransferase activity of SerC within the serine biosynthesis pathway, and we demonstrate that an mutant is a serine auxotroph, consistent with a function in l-serine biosynthesis. However, the mutant strain could produce SB when provided l-serine, suggesting that SbnI produces OPS for SB biosynthesis These findings indicate that besides transcriptionally regulating the locus, SbnI also has an enzymatic role in the SB biosynthetic pathway.

PubMed: 29483190
DOI: 10.1074/jbc.RA118.001875

実験手法

X-RAY DIFFRACTION (2.5 Å)