5UI7

Solution NMR Structure of Lasso Peptide Klebsidin

5UI7 の概要

• エントリーDOI: 10.2210/pdb5ui7/pdb
• 関連するPDBエントリー: 5UI6
• NMR情報: BMRB: 30230
• 分子名称: Klebsidin (1 entity in total)
• 機能のキーワード: lasso peptide rnap inhibitor, structure from cyana 2.1, antimicrobial protein
• 由来する生物種: Klebsiella pneumoniae 4541-2
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2052.25

構造登録者

Bushin, L.B.,Metelev, M.,Severinov, K.,Seyedsayamdost, M.R. (登録日: 2017-01-13, 公開日: 2017-02-08, 最終更新日: 2024-05-15)

主引用文献

Metelev, M.,Arseniev, A.,Bushin, L.B.,Kuznedelov, K.,Artamonova, T.O.,Kondratenko, R.,Khodorkovskii, M.,Seyedsayamdost, M.R.,Severinov, K.
Acinetodin and Klebsidin, RNA Polymerase Targeting Lasso Peptides Produced by Human Isolates of Acinetobacter gyllenbergii and Klebsiella pneumoniae.
ACS Chem. Biol., 12:814-824, 2017

PubMed Abstract: We report the bioinformatic prediction and structural validation of two lasso peptides, acinetodin and klebsidin, encoded by the genomes of several human-associated strains of Acinetobacter and Klebsiella. Computation of the three-dimensional structures of these peptides using NMR NOESY constraints verifies that they contain a lasso motif. Despite the lack of sequence similarity to each other or to microcin J25, a prototypical lasso peptide and transcription inhibitor from Escherichia coli, acinetodin and klebsidin also inhibit transcript elongation by the E. coli RNA polymerase by binding to a common site. Yet, unlike microcin J25, acinetodin and klebsidin are unable to permeate wild type E. coli cells and inhibit their growth. We show that the E. coli cells become sensitive to klebsidin when expressing the outer membrane receptor FhuA homologue from Klebsiella pneumoniae. It thus appears that specificity to a common target, the RNA polymerase secondary channel, can be attained by a surprisingly diverse set of primary sequences folded into a common threaded-lasso fold. In contrast, transport into cells containing sensitive targets appears to be much more specific and must be the major determinant of the narrow range of bioactivity of known lasso peptides.

PubMed: 28106375
DOI: 10.1021/acschembio.6b01154

実験手法

SOLUTION NMR