5UI5

Crystal structure of Aquifex aeolicus sigmaN bound to promoter DNA

Summary for 5UI5

DescriptorDNA (31-MER), DNA (30-MER), RNA polymerase sigma factor RpoN, ... (4 entities in total)
Functional Keywordshelix-turn-helix dna binding motif winged helix-turn-helix dna binding motif bacterial rna polymerase sigman bacterial rna polymerase sigma54, transcription-dna complex, transcription/dna
Biological sourceAquifex aeolicus (strain VF5)
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Total number of polymer chains6
Total molecular weight118877.68
Authors
Darst, S.A.,Campbell, E.A.,Rajashankar, K. (deposition date: 2017-01-12, release date: 2017-02-22, Last modification date: 2017-09-13)
Primary citation
Campbell, E.A.,Kamath, S.,Rajashankar, K.R.,Wu, M.,Darst, S.A.
Crystal structure of Aquifex aeolicus sigma (N) bound to promoter DNA and the structure of sigma (N)-holoenzyme.
Proc. Natl. Acad. Sci. U.S.A., 114:E1805-E1814, 2017
PubMed: 28223493 (PDB entries with the same primary citation)
DOI: 10.1073/pnas.1619464114
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.4 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.336390.2%12.0%3.5%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report