5UE0

1.90 A resolution structure of CT622 C-terminal domain from Chlamydia trachomatis

5UE0 の概要

• エントリーDOI: 10.2210/pdb5ue0/pdb
• 分子名称: CT622 protein, SULFATE ION (3 entities in total)
• 機能のキーワード: t3ss effector, helical bundle, prenylation, protein binding
• 由来する生物種: Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31848.35

構造登録者

Barta, M.L.,Lovell, S.,Battaile, K.P.,Hefty, P.S. (登録日: 2016-12-29, 公開日: 2018-01-10, 最終更新日: 2025-04-02)

主引用文献

Cosse, M.M.,Barta, M.L.,Fisher, D.J.,Oesterlin, L.K.,Niragire, B.,Perrinet, S.,Millot, G.A.,Hefty, P.S.,Subtil, A.
The Loss of Expression of a Single Type 3 Effector (CT622) Strongly ReducesChlamydia trachomatisInfectivity and Growth.
Front Cell Infect Microbiol, 8:145-145, 2018

PubMed Abstract: Invasion of epithelial cells by the obligate intracellular bacterium results in its enclosure inside a membrane-bound compartment termed an inclusion. The bacterium quickly begins manipulating interactions between host intracellular trafficking and the inclusion interface, diverging from the endocytic pathway and escaping lysosomal fusion. We have identified a previously uncharacterized protein, CT622, unique to the , in the absence of which most bacteria failed to establish a successful infection. CT622 is abundant in the infectious form of the bacteria, in which it associates with CT635, a putative novel chaperone protein. We show that CT622 is translocated into the host cytoplasm via type three secretion throughout the developmental cycle of the bacteria. Two separate domains of roughly equal size have been identified within CT622 and a 1.9 Å crystal structure of the C-terminal domain has been determined. Genetic disruption of expression resulted in a strong bacterial growth defect, which was due to deficiencies in proliferation and in the generation of infectious bacteria. Our results converge to identify CT622 as a secreted protein that plays multiple and crucial roles in the initiation and support of the growth cycle. They reveal that genetic disruption of a single effector can deeply affect bacterial fitness.

PubMed: 29868501
DOI: 10.3389/fcimb.2018.00145

実験手法

X-RAY DIFFRACTION (1.9 Å)