5U4V

pseudoGTPase domain (pG1) of p190RhoGAP-B

5U4V の概要

• エントリーDOI: 10.2210/pdb5u4v/pdb
• 関連するPDBエントリー: 5U4U
• 分子名称: Rho GTPase-activating protein 5 (2 entities in total)
• 機能のキーワード: gtpase, pseudogtpase, g domain, rho, gap, arhgap5, arhgap35, hydrolase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19843.62

構造登録者

Stiegler, A.L.,Boggon, T.J. (登録日: 2016-12-06, 公開日: 2017-09-20, 最終更新日: 2023-10-04)

主引用文献

Stiegler, A.L.,Boggon, T.J.
p190RhoGAP proteins contain pseudoGTPase domains.
Nat Commun, 8:506-506, 2017

PubMed Abstract: The two p190RhoGAP proteins, p190RhoGAP-A and -B, are key regulators of Rho GTPase signaling and are essential for actin cytoskeletal structure and contractility. Here we report the discovery of two evolutionarily conserved GTPase-like domains located in the 'middle domain', previously thought to be unstructured. Deletion of these domains reduces RhoGAP activity. Crystal structures, MANT-GTPγS binding, thermal denaturation, biochemical assays and sequence homology analysis all strongly support defects in nucleotide-binding activity. Analysis of p190RhoGAP proteins therefore indicates the presence of two previously unidentified domains which represent an emerging group of pseudoenzymes, the pseudoGTPases.A growing number of 'pseudoenzymes' with a regulatory role in signal transduction processes but without catalytic activity are being identified. Here, the authors identify two pseudoGTPase domains in p190RhoGAP, characterize them biochemically and structurally and show that they influence RhoGAP activity.

PubMed: 28894085
DOI: 10.1038/s41467-017-00483-x

実験手法

X-RAY DIFFRACTION (2.6 Å)