5U4K

NMR structure of the complex between the KIX domain of CBP and the transactivation domain 1 of p65

5U4K の概要

• エントリーDOI: 10.2210/pdb5u4k/pdb
• NMR情報: BMRB: 26867
• 分子名称: CREB-binding protein, Transcription factor p65 (2 entities in total)
• 機能のキーワード: transcription, p300-cbp coactivator family, nf-kb, transactivation domain
• 由来する生物種: Mus musculus (Mouse); 詳細
• 細胞内の位置: Nucleus: Q04206
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13732.45

構造登録者

Lecoq, L.,Raiola, L.,Chabot, P.R.,Cyr, N.,Arseneault, G.,Omichinski, J.G. (登録日: 2016-12-05, 公開日: 2017-03-08, 最終更新日: 2024-05-22)

主引用文献

Lecoq, L.,Raiola, L.,Chabot, P.R.,Cyr, N.,Arseneault, G.,Legault, P.,Omichinski, J.G.
Structural characterization of interactions between transactivation domain 1 of the p65 subunit of NF-kappa B and transcription regulatory factors.
Nucleic Acids Res., 45:5564-5576, 2017

PubMed Abstract: p65 is a member of the NF-κB family of transcriptional regulatory proteins that functions as the activating component of the p65-p50 heterodimer. Through its acidic transactivation domain (TAD), p65 has the capacity to form interactions with several different transcriptional regulatory proteins, including TFIIB, TFIIH, CREB-binding protein (CBP)/p300 and TAFII31. Like other acidic TADs, the p65 TAD contains two subdomains (p65TA1 and p65TA2) that interact with different regulatory factors depending on the target gene. Despite its role in controlling numerous NF-κB target genes, there are no high-resolution structures of p65TA1 bound to a target transcriptional regulatory factor. In this work, we characterize the interaction of p65TA1 with two factors, the Tfb1/p62 subunit of TFIIH and the KIX domain of CBP. In these complexes, p65TA1 transitions into a helical conformation that includes its characteristic ΦXXΦΦ motif (Φ = hydrophobic amino acid). Structural and functional studies demonstrate that the two binding interfaces are primarily stabilized by three hydrophobic amino acids within the ΦXXΦΦ motif and these residues are also crucial to its ability to activate transcription. Taken together, the results provide an atomic level description of how p65TA1 is able to bind different transcriptional regulatory factors needed to activate NF-κB target genes.

PubMed: 28334776
DOI: 10.1093/nar/gkx146

実験手法

SOLUTION NMR