5U3H

Solution structure of apo PCP1 from yersiniabactin synthetase

5U3H の概要

• エントリーDOI: 10.2210/pdb5u3h/pdb
• NMR情報: BMRB: 30205
• 分子名称: HMWP2 nonribosomal peptide synthetase (1 entity in total)
• 機能のキーワード: peptidyl carrier protein, nrps, ligase
• 由来する生物種: Yersinia pestis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12353.52

構造登録者

Harden, B.J.,Frueh, D.P. (登録日: 2016-12-02, 公開日: 2017-02-08, 最終更新日: 2024-05-15)

主引用文献

Harden, B.J.,Frueh, D.P.
Molecular Cross-Talk between Nonribosomal Peptide Synthetase Carrier Proteins and Unstructured Linker Regions.
Chembiochem, 18:629-632, 2017

PubMed Abstract: Nonribosomal peptide synthetases (NRPSs) employ multiple domains separated by linker regions to incorporate substrates into natural products. During synthesis, substrates are covalently tethered to carrier proteins that translocate between catalytic partner domains. The molecular parameters that govern translocation and associated linker remodeling remain unknown. Here, we used NMR to characterize the structure, dynamics, and invisible states of a peptidyl carrier protein flanked by its linkers. We showed that the N-terminal linker stabilizes and interacts with the protein core while modulating dynamics at specific sites involved in post-translational modifications and/or domain interactions. The results detail the molecular communication between peptidyl carrier proteins and their linkers and could guide efforts in engineering NRPSs to obtain new pharmaceuticals.

PubMed: 28120469
DOI: 10.1002/cbic.201700030

実験手法

SOLUTION NMR