5TY4

MicroED structure of a complex between monomeric TGF-b and its receptor, TbRII, at 2.9 A resolution

5TY4 の概要

• エントリーDOI: 10.2210/pdb5ty4/pdb
• 関連するPDBエントリー: 5K7N 5K7O 5K7P 5K7Q 5K7R 5K7S 5K7T
• EMDBエントリー: 8216 8217 8218 8219 8220 8221 8222 8472
• 分子名称: TGF-beta receptor type-2, mmTGF-b2-7m (2 entities in total)
• 機能のキーワード: transferase
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22865.33

構造登録者

Weiss, S.C.,de la Cruz, M.J.,Hattne, J.,Shi, D.,Reyes, F.E.,Callero, G.,Gonen, T. (登録日: 2016-11-18, 公開日: 2017-04-26, 最終更新日: 2024-11-06)

主引用文献

de la Cruz, M.J.,Hattne, J.,Shi, D.,Seidler, P.,Rodriguez, J.,Reyes, F.E.,Sawaya, M.R.,Cascio, D.,Weiss, S.C.,Kim, S.K.,Hinck, C.S.,Hinck, A.P.,Calero, G.,Eisenberg, D.,Gonen, T.
Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.
Nat. Methods, 14:399-402, 2017

PubMed Abstract: Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.

PubMed: 28192420
DOI: 10.1038/nmeth.4178

実験手法

ELECTRON CRYSTALLOGRAPHY (2.9 Å)