5TXZ

DNA Polymerase Mu Reactant Complex, 100mM Mg2+ (15 min)

5TXZ の概要

• エントリーDOI: 10.2210/pdb5txz/pdb
• 関連するPDBエントリー: 5TXX 5TYB 5TYC 5TYD 5TYE 5TYF 5TYG 5TYU 5TYV 5TYW 5TYX 5TYY 5TYZ
• 分子名称: DNA-directed DNA/RNA polymerase mu, 1,2-ETHANEDIOL, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, ... (13 entities in total)
• 機能のキーワード: time-lapse crystallography, product metal, dna polymerase mu, double strand break repair, transferase-dna complex, transferase/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus : Q9NP87
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 47080.94

構造登録者

Jamsen, J.A.,Wilson, S.H. (登録日: 2016-11-17, 公開日: 2017-08-30, 最終更新日: 2024-10-30)

主引用文献

Jamsen, J.A.,Beard, W.A.,Pedersen, L.C.,Shock, D.D.,Moon, A.F.,Krahn, J.M.,Bebenek, K.,Kunkel, T.A.,Wilson, S.H.
Time-lapse crystallography snapshots of a double-strand break repair polymerase in action.
Nat Commun, 8:253-253, 2017

PubMed Abstract: DNA polymerase (pol) μ is a DNA-dependent polymerase that incorporates nucleotides during gap-filling synthesis in the non-homologous end-joining pathway of double-strand break repair. Here we report time-lapse X-ray crystallography snapshots of catalytic events during gap-filling DNA synthesis by pol μ. Unique catalytic intermediates and active site conformational changes that underlie catalysis are uncovered, and a transient third (product) metal ion is observed in the product state. The product manganese coordinates phosphate oxygens of the inserted nucleotide and PP. The product metal is not observed during DNA synthesis in the presence of magnesium. Kinetic analyses indicate that manganese increases the rate constant for deoxynucleoside 5'-triphosphate insertion compared to magnesium. The likely product stabilization role of the manganese product metal in pol μ is discussed. These observations provide insight on structural attributes of this X-family double-strand break repair polymerase that impact its biological function in genome maintenance.DNA polymerase (pol) μ functions in DNA double-strand break repair. Here the authors use time-lapse X-ray crystallography to capture the states of pol µ during the conversion from pre-catalytic to product complex and observe a third transiently bound metal ion in the product state.

PubMed: 28811466
DOI: 10.1038/s41467-017-00271-7

実験手法

X-RAY DIFFRACTION (1.651 Å)