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5TW1

Crystal structure of a Mycobacterium smegmatis transcription initiation complex with RbpA

Summary for 5TW1
Entry DOI10.2210/pdb5tw1/pdb
DescriptorRNA polymerase-binding protein RbpA, SULFATE ION, 1,2-ETHANEDIOL, ... (14 entities in total)
Functional Keywordstranscription activator-transferase-dna complex, transcription activator/transferase/dna
Biological sourceMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
More
Total number of polymer chains11
Total formula weight486108.83
Authors
Hubin, E.A.,Darst, S.A.,Campbell, E.A. (deposition date: 2016-11-10, release date: 2017-01-18, Last modification date: 2024-12-25)
Primary citationHubin, E.A.,Fay, A.,Xu, C.,Bean, J.M.,Saecker, R.M.,Glickman, M.S.,Darst, S.A.,Campbell, E.A.
Structure and function of the mycobacterial transcription initiation complex with the essential regulator RbpA.
Elife, 6:-, 2017
Cited by
PubMed Abstract: RbpA and CarD are essential transcription regulators in mycobacteria. Mechanistic analyses of promoter open complex (RPo) formation establish that RbpA and CarD cooperatively stimulate formation of an intermediate (RP2) leading to RPo; formation of RP2 is likely a bottleneck step at the majority of mycobacterial promoters. Once RPo forms, CarD also disfavors its isomerization back to RP2. We determined a 2.76 Å-resolution crystal structure of a mycobacterial transcription initiation complex (TIC) with RbpA as well as a CarD/RbpA/TIC model. Both CarD and RbpA bind near the upstream edge of the -10 element where they likely facilitate DNA bending and impede transcription bubble collapse. In vivo studies demonstrate the essential role of RbpA, show the effects of RbpA truncations on transcription and cell physiology, and indicate additional functions for RbpA not evident in vitro. This work provides a framework to understand the control of mycobacterial transcription by RbpA and CarD.
PubMed: 28067618
DOI: 10.7554/eLife.22520
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.76 Å)
Structure validation

238582

數據於2025-07-09公開中

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