5TUJ

Ancestral Cationic Amino Acid Solute Binding Protein (AncCDT-1)

5TUJ の概要

• エントリーDOI: 10.2210/pdb5tuj/pdb
• 分子名称: Ancestral protein CDT-Anc1 (1 entity in total)
• 機能のキーワード: periplasmic solute binding protein, solute binding protein
• 由来する生物種: unidentified
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26292.08

構造登録者

Kaczmarski, J.A.,Clifton, B.E.,Carr, P.D.,Jackson, C.J. (登録日: 2016-11-06, 公開日: 2017-12-06, 最終更新日: 2024-03-06)

主引用文献

Clifton, B.E.,Kaczmarski, J.A.,Carr, P.D.,Gerth, M.L.,Tokuriki, N.,Jackson, C.J.
Evolution of cyclohexadienyl dehydratase from an ancestral solute-binding protein.
Nat. Chem. Biol., 14:542-547, 2018

PubMed Abstract: The emergence of enzymes through the neofunctionalization of noncatalytic proteins is ultimately responsible for the extraordinary range of biological catalysts observed in nature. Although the evolution of some enzymes from binding proteins can be inferred by homology, we have a limited understanding of the nature of the biochemical and biophysical adaptations along these evolutionary trajectories and the sequence in which they occurred. Here we reconstructed and characterized evolutionary intermediate states linking an ancestral solute-binding protein to the extant enzyme cyclohexadienyl dehydratase. We show how the intrinsic reactivity of a desolvated general acid was harnessed by a series of mutations radiating from the active site, which optimized enzyme-substrate complementarity and transition-state stabilization and minimized sampling of noncatalytic conformations. Our work reveals the molecular evolutionary processes that underlie the emergence of enzymes de novo, which are notably mirrored by recent examples of computational enzyme design and directed evolution.

PubMed: 29686357
DOI: 10.1038/s41589-018-0043-2

実験手法

X-RAY DIFFRACTION (3.352 Å)