5TTK

Crystal Structure of Selenomethionine-incorporated Nicotine Oxidoreductase from Pseudomonas putida

5TTK の概要

• エントリーDOI: 10.2210/pdb5ttk/pdb
• 関連するPDBエントリー: 5TTJ
• 分子名称: Amine oxidase, FLAVIN-ADENINE DINUCLEOTIDE (3 entities in total)
• 機能のキーワード: nicotine degradation, flavoenzyme, monoamine oxidase family, phbh, oxidoreductase
• 由来する生物種: Pseudomonas putida (strain S16)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 219679.56

構造登録者

Tararina, M.A.,Janda, K.D.,Allen, K.N. (登録日: 2016-11-03, 公開日: 2017-01-25, 最終更新日: 2024-11-20)

主引用文献

Tararina, M.A.,Janda, K.D.,Allen, K.N.
Structural Analysis Provides Mechanistic Insight into Nicotine Oxidoreductase from Pseudomonas putida.
Biochemistry, 55:6595-6598, 2016

PubMed Abstract: The first structure of nicotine oxidoreductase (NicA2) was determined by X-ray crystallography. Pseudomonas putida has evolved nicotine-degrading activity to provide a source of carbon and nitrogen. The structure establishes NicA2 as a member of the monoamine oxidase family. Residues 1-50 are disordered and may play a role in localization. The nicotine-binding site proximal to the isoalloxazine ring of flavin shows an unusual composition of the classical aromatic cage (W427 and N462). The active site architecture is consistent with the proposed binding of the deprotonated form of the substrate and the flavin-dependent oxidation of the pyrrolidone C-N bond followed by nonenzymatic hydrolysis.

PubMed: 27933790
DOI: 10.1021/acs.biochem.6b00963

実験手法

X-RAY DIFFRACTION (2.51 Å)