5TLC

Crystal structure of BdsA from Bacillus subtilis WU-S2B

5TLC の概要

• エントリーDOI: 10.2210/pdb5tlc/pdb
• 分子名称: Dibenzothiophene desulfurization enzyme A (2 entities in total)
• 機能のキーワード: monooxygenase, oxidoreductase
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 198838.92

構造登録者

Okai, M.,Lee, W.C.,Tanokura, M. (登録日: 2016-10-11, 公開日: 2017-05-03, 最終更新日: 2024-03-20)

主引用文献

Okai, M.,Lee, W.C.,Guan, L.J.,Ohshiro, T.,Izumi, Y.,Tanokura, M.
Crystal structure of dibenzothiophene sulfone monooxygenase BdsA from Bacillus subtilis WU-S2B
Proteins, 85:1171-1177, 2017

PubMed Abstract: The dibenzothiophene (DBT) sulfone monooxygenase BdsA from Bacillus subtilis WU-S2B catalyzes the conversion of DBT sulfone to 2'-hydroxybiphenyl 2-sulfinate. We report the crystal structures of BdsA at a resolution of 2.80 Å. BdsA exists as a homotetramer with a dimer-of-dimers configuration in the crystal, and the interaction between E288 and R296 in BdsA is important for tetramer formation. A structural comparison with homologous proteins shows that the orientation and location of the α9-α12 helices in BdsA are closer to those of the closed form than those of the open form in the EDTA monooxygenase EmoA. Proteins 2017; 85:1171-1177. © 2017 Wiley Periodicals, Inc.

PubMed: 28205250
DOI: 10.1002/prot.25267

実験手法

X-RAY DIFFRACTION (2.8 Å)