5TJ6

Ca2+ bound aplysia Slo1

5TJ6 の概要

• エントリーDOI: 10.2210/pdb5tj6/pdb
• 関連するPDBエントリー: 5JTI
• EMDBエントリー: 8410 8414
• 分子名称: High conductance calcium-activated potassium channel, POTASSIUM ION, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: ion channel, k+ channel, ca2+ bound, high conductance, membrane protein
• 由来する生物種: Aplysia californica (California sea hare)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 131843.06

構造登録者

MacKinnon, R.,Tao, X.,Hite, R.K. (登録日: 2016-10-03, 公開日: 2016-12-14, 最終更新日: 2024-03-13)

主引用文献

Tao, X.,Hite, R.K.,MacKinnon, R.
Cryo-EM structure of the open high-conductance Ca(2+)-activated K(+) channel.
Nature, 541:46-51, 2017

PubMed Abstract: The Ca-activated K channel, Slo1, has an unusually large conductance and contains a voltage sensor and multiple chemical sensors. Dual activation by membrane voltage and Ca renders Slo1 central to processes that couple electrical signalling to Ca-mediated events such as muscle contraction and neuronal excitability. Here we present the cryo-electron microscopy structure of a full-length Slo1 channel from Aplysia californica in the presence of Ca and Mg at a resolution of 3.5 Å. The channel adopts an open conformation. Its voltage-sensor domain adopts a non-domain-swapped attachment to the pore and contacts the cytoplasmic Ca-binding domain from a neighbouring subunit. Unique structural features of the Slo1 voltage sensor suggest that it undergoes different conformational changes than other known voltage sensors. The structure reveals the molecular details of three distinct divalent cation-binding sites identified through electrophysiological studies of mutant Slo1 channels.

PubMed: 27974795
DOI: 10.1038/nature20608

実験手法

ELECTRON MICROSCOPY (3.5 Å)