5TIK

Structural basis for inhibition of erythrocyte invasion by antibodies to Plasmodium falciparum protein CyRPA

5TIK の概要

• エントリーDOI: 10.2210/pdb5tik/pdb
• 関連するPDBエントリー: 5TIH
• 分子名称: Cysteine-rich protective antigen (2 entities in total)
• 機能のキーワード: plasmodium falciparum cyrpa inhibitory antibody, immune system
• 由来する生物種: Plasmodium falciparum
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 157941.61

構造登録者

Chen, L.,Xu, Y.,Wang, W.,Thompson, J.K.,Goddard-Borger, E.,Lawrence, M.C.,Cowman, A.F. (登録日: 2016-10-03, 公開日: 2017-03-01, 最終更新日: 2024-11-06)

主引用文献

Chen, L.,Xu, Y.,Wong, W.,Thompson, J.K.,Healer, J.,Goddard-Borger, E.D.,Lawrence, M.C.,Cowman, A.F.
Structural basis for inhibition of erythrocyte invasion by antibodies toPlasmodium falciparumprotein CyRPA.
Elife, 6:-, 2017

PubMed Abstract: causes malaria in humans with over 450,000 deaths annually. The asexual blood stage involves invasion of erythrocytes by merozoites, in which they grow and divide to release daughter merozoites, which in turn invade new erythrocytes perpetuating the cycle responsible for malaria. A key step in merozoite invasion is the essential binding of PfRh5/CyRPA/PfRipr complex to basigin, a step linked to the formation of a pore between merozoites and erythrocytes. We show CyRPA interacts directly with PfRh5. An invasion inhibitory monoclonal antibody to CyRPA blocks binding of CyRPA to PfRh5 and complex formation thus illuminating the molecular mechanism for inhibition of parasite growth. We determined the crystal structures of CyRPA alone and in complex with an antibody Fab fragment. CyRPA has a six-bladed β-propeller fold, and we identify the region that interacts with PfRh5. This functionally conserved epitope is a potential target for vaccines against .

PubMed: 28195530
DOI: 10.7554/eLife.21347

実験手法

X-RAY DIFFRACTION (3.09 Å)