5TI8

Crystal Structure of an aspartate aminotransferase from Pseudomonas

5TI8 の概要

• エントリーDOI: 10.2210/pdb5ti8/pdb
• 分子名称: Aminotransferase, CALCIUM ION (3 entities in total)
• 機能のキーワード: aminotransferase, transferase
• 由来する生物種: Pseudomonas sp. (strain M1)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 104927.75

構造登録者

Peat, T.S.,Newman, J. (登録日: 2016-10-01, 公開日: 2017-01-11, 最終更新日: 2023-10-04)

主引用文献

Wilding, M.,Scott, C.,Newman, J.,Peat, T.S.
Crystal structure of a putrescine aminotransferase from Pseudomonas sp. strain AAC.
Acta Crystallogr F Struct Biol Commun, 73:29-35, 2017

PubMed Abstract: The putrescine aminotransferase KES24511 from Pseudomonas sp. strain AAC was previously identified as an industrially relevant enzyme based on the discovery that it is able to promiscuously catalyse the transamination of 12-aminododecanoic acid. Here, the cloning, heterologous expression, purification and successful crystallization of the KES24511 protein are reported, which ultimately generated crystals adopting space group I2. The crystals diffracted X-rays to 2.07 Å resolution and data were collected using the microfocus beamline of the Australian Synchrotron. The structure was solved using molecular replacement, with a monomer from PDB entry 4a6t as the search model.

PubMed: 28045391
DOI: 10.1107/S2053230X16019658

実験手法

X-RAY DIFFRACTION (2.07 Å)