5TE6

Crystal Structure of Broadly Neutralizing VRC01-class Antibody N6 in Complex with HIV-1 Clade AE Strain 93TH057 gp120 Core

5TE6 の概要

• エントリーDOI: 10.2210/pdb5te6/pdb
• 関連するPDBエントリー: 5TE4 5TE7
• 分子名称: clade A/E 93TH057 HIV-1 gp120 core, Heavy chain of N6, Light chain of N6, ... (6 entities in total)
• 機能のキーワード: hiv-1, vrc01-class antibody, n6, cd4-binding site, gp120, immune system
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 89236.02

構造登録者

Zhou, T.,Kwong, P.D. (登録日: 2016-09-20, 公開日: 2016-11-16, 最終更新日: 2024-11-20)

主引用文献

Huang, J.,Kang, B.H.,Ishida, E.,Zhou, T.,Griesman, T.,Sheng, Z.,Wu, F.,Doria-Rose, N.A.,Zhang, B.,McKee, K.,O'Dell, S.,Chuang, G.Y.,Druz, A.,Georgiev, I.S.,Schramm, C.A.,Zheng, A.,Joyce, M.G.,Asokan, M.,Ransier, A.,Darko, S.,Migueles, S.A.,Bailer, R.T.,Louder, M.K.,Alam, S.M.,Parks, R.,Kelsoe, G.,Von Holle, T.,Haynes, B.F.,Douek, D.C.,Hirsch, V.,Seaman, M.S.,Shapiro, L.,Mascola, J.R.,Kwong, P.D.,Connors, M.
Identification of a CD4-Binding-Site Antibody to HIV that Evolved Near-Pan Neutralization Breadth.
Immunity, 45:1108-1121, 2016

PubMed Abstract: Detailed studies of the broadly neutralizing antibodies (bNAbs) that underlie the best available examples of the humoral immune response to HIV are providing important information for the development of therapies and prophylaxis for HIV-1 infection. Here, we report a CD4-binding site (CD4bs) antibody, named N6, that potently neutralized 98% of HIV-1 isolates, including 16 of 20 that were resistant to other members of its class. N6 evolved a mode of recognition such that its binding was not impacted by the loss of individual contacts across the immunoglobulin heavy chain. In addition, structural analysis revealed that the orientation of N6 permitted it to avoid steric clashes with glycans, which is a common mechanism of resistance. Thus, an HIV-1-specific bNAb can achieve potent, near-pan neutralization of HIV-1, making it an attractive candidate for use in therapy and prophylaxis.

PubMed: 27851912
DOI: 10.1016/j.immuni.2016.10.027

実験手法

X-RAY DIFFRACTION (2.4 Å)