5TE5

Crystal structure of Bos taurus opsin regenerated with 6-carbon ring retinal chromophore

5TE5 の概要

• エントリーDOI: 10.2210/pdb5te5/pdb
• 関連するPDBエントリー: 5TE3
• 分子名称: Rhodopsin, (2E)-{(4E)-4-[(3E)-4-(2,6,6-trimethylcyclohex-1-en-1-yl)but-3-en-2-ylidene]cyclohex-2-en-1-ylidene}acetaldehyde (2 entities in total)
• 機能のキーワード: signaling protein
• 由来する生物種: Bos taurus (Bovine)
• 細胞内の位置: Membrane; Multi-pass membrane protein: P02699
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39353.94

構造登録者

Gulati, S.,Banerjee, S.,Katayama, K.,Kiser, P.D.,Palczewski, K. (登録日: 2016-09-20, 公開日: 2017-03-15, 最終更新日: 2024-11-20)

主引用文献

Gulati, S.,Jastrzebska, B.,Banerjee, S.,Placeres, A.L.,Miszta, P.,Gao, S.,Gunderson, K.,Tochtrop, G.P.,Filipek, S.,Katayama, K.,Kiser, P.D.,Mogi, M.,Stewart, P.L.,Palczewski, K.
Photocyclic behavior of rhodopsin induced by an atypical isomerization mechanism.
Proc. Natl. Acad. Sci. U.S.A., 114:E2608-E2615, 2017

PubMed Abstract: Vertebrate rhodopsin (Rh) contains 11--retinal as a chromophore to convert light energy into visual signals. On absorption of light, 11--retinal is isomerized to all--retinal, constituting a one-way reaction that activates transducin (G) followed by chromophore release. Here we report that bovine Rh, regenerated instead with a six-carbon-ring retinal chromophore featuring a C=C double bond locked in its conformation (Rh6mr), employs an atypical isomerization mechanism by converting 11- to an 11,13- configuration for prolonged G activation. Time-dependent UV-vis spectroscopy, HPLC, and molecular mechanics analyses revealed an atypical thermal reisomerization of the 11,13- to the 11- configuration on a slow timescale, which enables Rh6mr to function in a photocyclic manner similar to that of microbial Rhs. With this photocyclic behavior, Rh6mr repeatedly recruits and activates G in response to light stimuli, making it an excellent candidate for optogenetic tools based on retinal analog-bound vertebrate Rhs. Overall, these comprehensive structure-function studies unveil a unique photocyclic mechanism of Rh activation by an 11--to-11,13- isomerization.

PubMed: 28289214
DOI: 10.1073/pnas.1617446114

実験手法

X-RAY DIFFRACTION (4.01 Å)