5TCL
Thaumatin from 4.96 A wavelength data collection
Summary for 5TCL
| Entry DOI | 10.2210/pdb5tcl/pdb |
| Descriptor | Thaumatin-1, L(+)-TARTARIC ACID, GLYCEROL (3 entities in total) |
| Functional Keywords | long wavelength, s-sad, vacuum, plant protein |
| Biological source | Thaumatococcus daniellii (Katemfe) |
| Cellular location | Cytoplasmic vesicle: P02883 |
| Total number of polymer chains | 1 |
| Total formula weight | 22469.24 |
| Authors | Aurelius, O.,Duman, R.,El Omari, K.,Mykhaylyk, V.,Wagner, A. (deposition date: 2016-09-15, release date: 2017-09-20, Last modification date: 2024-11-13) |
| Primary citation | Aurelius, O.,Duman, R.,El Omari, K.,Mykhaylyk, V.,Wagner, A. Long-wavelength macromolecular crystallography - First successful native SAD experiment close to the sulfur edge. Nucl Instrum Methods Phys Res B, 411:12-16, 2017 Cited by PubMed Abstract: Phasing of novel macromolecular crystal structures has been challenging since the start of structural biology. Making use of anomalous diffraction of natively present elements, such as sulfur and phosphorus, for phasing has been possible for some systems, but hindered by the necessity to access longer X-ray wavelengths in order to make most use of the anomalous scattering contributions of these elements. Presented here are the results from a first successful experimental phasing study of a macromolecular crystal structure at a wavelength close to the sulfur K edge. This has been made possible by the in-vacuum setup and the long-wavelength optimised experimental setup at the I23 beamline at Diamond Light Source. In these early commissioning experiments only standard data collection and processing procedures have been applied, in particular no dedicated absorption correction has been used. Nevertheless the success of the experiment demonstrates that the capability to extract phase information can be even further improved once data collection protocols and data processing have been optimised. PubMed: 29276323DOI: 10.1016/j.nimb.2016.12.005 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.201 Å) |
Structure validation
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