Loading
PDBj
メニューPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

5T5F

Neisseria meningitidis factor H binding protein in complex with monoclonal antibody JAR5

5T5F の概要
エントリーDOI10.2210/pdb5t5f/pdb
分子名称Factor H binding protein variant B24, Monoclonal antibody Jar5 heavy chain, Monoclonal antibody Jar5 light chain, ... (4 entities in total)
機能のキーワードfhbp, jar5, epitope mapping, monoclonal antibody, cooperativity, neisseria meningitidis, protein binding
由来する生物種Neisseria meningitidis
詳細
タンパク質・核酸の鎖数3
化学式量合計73908.72
構造登録者
Malito, E. (登録日: 2016-08-30, 公開日: 2016-11-09, 最終更新日: 2024-11-13)
主引用文献Malito, E.,Lo Surdo, P.,Veggi, D.,Santini, L.,Stefek, H.,Brunelli, B.,Luzzi, E.,Bottomley, M.J.,Beernink, P.T.,Scarselli, M.
Neisseria meningitidis factor H-binding protein bound to monoclonal antibody JAR5: implications for antibody synergy.
Biochem. J., 473:4699-4713, 2016
Cited by
PubMed Abstract: Factor H-binding protein (fHbp) is an important antigen of Neisseria meningitidis that is capable of eliciting a robust protective immune response in humans. Previous studies on the interactions of fHbp with antibodies revealed that some anti-fHbp monoclonal antibodies that are unable to trigger complement-mediated bacterial killing in vitro are highly co-operative and become bactericidal if used in combination. Several factors have been shown to influence such co-operativity, including IgG subclass and antigen density. To investigate the structural basis of the anti-fHbp antibody synergy, we determined the crystal structure of the complex between fHbp and the Fab (fragment antigen-binding) fragment of JAR5, a specific anti-fHbp murine monoclonal antibody known to be highly co-operative with other monoclonal antibodies. We show that JAR5 is highly synergic with monoclonal antibody (mAb) 12C1, whose structure in complex with fHbp has been previously solved. Structural analyses of the epitopes recognized by JAR5 and 12C1, and computational modeling of full-length IgG mAbs of JAR5 and 12C1 bound to the same fHbp molecule, provide insights into the spatial orientation of Fc (fragment crystallizable) regions and into the possible implications for the susceptibility of meningococci to complement-mediated killing.
PubMed: 27784765
DOI: 10.1042/BCJ20160806
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.98 Å)
構造検証レポート
Validation report summary of 5t5f
検証レポート(詳細版)ダウンロードをダウンロード

239149

件を2025-07-23に公開中

PDB statisticsPDBj update infoContact PDBjnumon