5T56

[3]catenane from MccJ25 G12R/I13C/G21C lasso peptide

5T56 の概要

• エントリーDOI: 10.2210/pdb5t56/pdb
• NMR情報: BMRB: 30165
• 分子名称: Microcin J25 (2 entities in total)
• 機能のキーワード: catenane, lasso peptide, de novo protein
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Secreted: Q9X2V7 Q9X2V7
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 4561.16

構造登録者

Link, A.J.,Allen, C.D. (登録日: 2016-08-30, 公開日: 2017-07-12, 最終更新日: 2024-11-13)

主引用文献

Allen, C.D.,Link, A.J.
Self-Assembly of Catenanes from Lasso Peptides.
J. Am. Chem. Soc., 138:14214-14217, 2016

PubMed Abstract: Lasso peptides exist naturally in a threaded state as [1]rotaxanes, and we reasoned that lasso peptides cleaved in their loop region could serve as building blocks for catenanes. Mutagenesis of the lasso peptide microcin J25 (MccJ25) with two cysteine residues followed by cleavage of the peptide with trypsin led to a [2]rotaxane structure that self-assembled into a [3]catenane and [4]catenanes at room temperature in aqueous solution. The [3]catenane represents the smallest ring size of a catenane composed solely of polypeptide segments. The NMR structure of the [3]catenane was determined, suggesting that burial of hydrophobic residues may be a driving force for assembly of the catenane structure.

PubMed: 27768305
DOI: 10.1021/jacs.6b09454

実験手法

SOLUTION NMR